Jian Li


Jian Li    Assistant Professor, PI
InstituteSchool of Physical Science and Technology
Research AreaCell-free Protein Synthesis (CFPS), Natural Product Biosynthesis, Synthetic Biology, Biocatalysis
Contact Info.lijian@@shanghaitech.edu.cn

Dr. Jian Li received his PhD degree in Biotechnology from Technische Universität Berlin, Germany, in 2013. He continued to work as a postdoctoral associate at Northwestern University, USA, from 2013 to 2017. Since September 2017, he became an Assistant Professor, PI, at the School of Physical Science and Technology, ShanghaiTech University.

Research Interests

1. Cell-free synthetic biology;

2. Development of novel cell-free protein synthesis (CFPS) systems;

3. Natural product biosynthesis and discovery;

4. Biocatalysis and biotransformation;

5. Novel protein-based biomaterials.

Selected Publications

(*Corresponding author, #Co-first author)

21. Yang C, Liu Y, Liu WQ, Wu C, Li J*. Designing modular cell-free systems for tunable biotransformation of L-phenylalanine to aromatic compounds. Frontiers in Bioengineering and Biotechnology, 2021, 9, 730663

20. Huang S#, Liu Y#, Liu WQ, Neubauer P*, Li J*. The nonribosomal peptide valinomycin: From discovery to bioactivity and biosynthesis. Microorganisms, 2021, 9, 780

19. Feng J#, Yang C#, Zhao Z, Xu J, Li J*, Li P*. Application of cell-free protein synthesis system for the biosynthesis of L-theanine. ACS Synthetic Biology, 202110, 620–631

18. Liu WQ, Wu C*, Jewett MC, Li J*. Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system. Biotechnology and Bioengineering, 2020, 117, 4001–4008

17. Zhang L, Liu WQ, Li J*. Establishing a eukaryotic Pichia pastoris cell-free protein synthesis system. Frontiers in Bioengineering and Biotechnology, 2020, 8, 536

16. Xu H, Liu WQ, Li J*. Translation related factors improve the productivity of a Streptomyces-based cell-free protein synthesis system. ACS Synthetic Biology, 2020, 9, 1221–1224

15. Zhuang L#, Huang S#, Liu WQ, Karim AS, Jewett MC*, Li J*. Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin. Metabolic Engineering, 202060, 37–44

14. Liu WQ#, Zhang L#, Chen M#, Li J*. Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications. Biochemical Engineering Journal, 2019, 141, 182–189

13. Li J*, Zhang L, Liu WQ. Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products. Synthetic and Systems Biotechnology, 2018, 3, 83–89

12.Wang H, Li J*, Jewett MC*. Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements. Synthetic Biology, 2018, 3, ysy003

PhD and Postdoc publications

11.Casini A, Chang FY, Eluere R, King A, Young EM, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Espah Borujeni A, Ryu MH, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O'Connor S, Medema MH, Fischbach M, Jewett MC, Voigt CA, Gordon DB. A pressure test to make 10 molecules in 90 days: external evaluation of methods to engineer biology. Journal of the American Chemical Society, 2018, 140, 4302–4316

10. Li J, Wang H, Jewett MC. Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields. Biochemical Engineering Journal, 2018, 130, 29–33

9. Li J, Wang H, Kwon YC, Jewett MC. Establishing a high yielding Streptomyces-based cell-free protein synthesis system. Biotechnology and Bioengineering, 2017, 114, 1343–1353 (Video Highlighted)

8. Goering AW#, Li J#, McClure RA, Thomson RJ, Jewett MC, Kelleher NL. In vitro reconstruction of nonribosomal peptide biosynthesis directly from DNA using cell-free protein synthesis. ACS Synthetic Biology, 2017, 6, 39–44

7. Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, Jewett MC. Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase. Biotechnology Journal, 2016, 11, 212–218 (Front Cover)

6. Moatsou D#, Li J#, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK. Self-assembly of temperature-responsive protein-polymer bioconjugates. Bioconjugate Chemistry, 2015, 26, 1890–1899 (Front Cover)

5. Li J*, Jaitzig J, Lu P, Süssmuth RD, Neubauer P. Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations. Microbial Cell Factories, 2015, 14, 83

4. Li J*, Jaitzig J, Theuer L, Legala OE, Süssmuth RD, Neubauer P. Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli. Journal of Biotechnology, 2015, 193, 16–22

3. Li J*, Neubauer P. Escherichia coli as a cell factory for heterologous production of nonribosomal peptides and polyketides. New Biotechnology, 2014, 31, 579–585

2. Li J#, Jaitzig J#, Hillig F, Süssmuth RD, Neubauer P. Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation. Applied Microbiology and Biotechnology, 2014, 98, 591–601

1. Jaitzig J#, Li J#, Süssmuth RD, Neubauer P. Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli. ACS Synthetic Biology, 2014, 3, 432–438