刘一凡

课题组长

刘一凡，助理教授，博导，研究员    通讯地址：上海科技大学物质学院3号楼310-1    电子邮件：liuyf6@@shanghaitech.edu.cn    ORCID: 0000-0002-2989-6280 2007.09-2011.06    上海交通大学，生物医学工程，学士 2009.08-2009.12    香港理工大学，生物医学工程，交换生 2011.08-2016.01    香港科技大学，电子与计算机工程，博士 2016.03-2017.12    美国加州大学旧金山分校，生物工程，博士后 2018.11 至今          上海科技大学物质学院，助理教授，博导，研究员 个人荣誉：2024年上海市东方英才（青年），2023年上海市青年科技启明星、浦东明珠人才，2022年上科大优秀教师 教授课程：    秋学期：软物质微纳加工（含实验，6学分本研一体课程，与王宏达老师共同授课）    春学期： 生物医学微纳器件（2学分本研一体课程）

研究方向

本课题组（高通量生物技术实验室）致力于运用生物-信息交叉（BT-IT）手段，开发新的高通量生物技术和方法，为解析生命科学和医学领域中的复杂系统提供新的使能方案。

本课题组的研究内容主要结合兴趣以及国家重大科学需求。最近我们的研究方向为：

1）微生物高通量单细胞组学技术开发;

2）基于微生物单细胞组学的基因挖掘 (Genome-resolved gene mining);

3）高通量筛选和检测技术、高通量微流控技术开发。

科研成果

※研究论文

37. Mengzhen Li#, Xuanpei Zhai#, Jie Li#, Shiyan Li, Yanan Du, Jian Zhang, Rong Zhang, Yuan Luo, Wu Wei*, Yifan Liu*.

High-coverage, massively parallel sequencing of single-cell genomes with CAP-seq.

(BioRxiv)

36. Huixian Lin, Bo Li, Jingyun Guo, Xueying Mai, Haiyang Yu, Weilun Pan, Bodeng Wu, Wei Liu, Mingzhen Zhong, Tong Liao, Ye Zhang, Bo Situ, Xiaohui Yan, Yifan Liu*, Chunchen Liu*, Lei Zheng*.

Simultaneous detection of membrane protein and mRNA at single extracellular vesicle level by droplet microfluidics for cancer diagnosis.

Journal of Advanced Research, 2024.

35. Jiawei Zhu#, Yaru Meng#, Wenli Gao#, Shuo Yang, Wenjie Zhu, Xiangyang Ji, Xuanpei Zhai, Wan-Qiu Liu, Yuan Luo, Shengjie Ling*, Jian Li*, Yifan Liu*.

AI-driven high-throughput droplet screening of cell-free gene expression.

Nature Communications, revised (BioRxiv). 

34. Wan-Qiu Liu#, Xiangyang Ji#, Fang Ba, Yufei Zhang, Huiling Xu, Shuhui Huang, Xiao Zheng, Yifan Liu*, Shengjie Ling*, Michael C. Jewett*, Jian Li*. 

Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides.

Nature Communications, 2024.

33. Xiaorong Pan, Gaowei Lei, Jie Li, Tongshu Luan, Yiqing Hu, Yuanyuan Chu, Yu Feng, Wenrong Zhan, Chunxia Zhao Frederic A. Meunier, Yifan Liu, Yi Li*, Tong Wang*.

Actomyosin-II proactively shields axons of the central nervous system from mild mechanical stress.

Journal of Cell Biology, 2024.

32. Zheyi Jiang#, Meng Wei#, Jiawei Zhu#, Chengguang Wang, Tiantian Zhang, Rong Zhang, Peng Zhang, Alex Chia Yu Chang*, Yifan Liu*, Junfeng Zhang*.

NebulaPlate: a droplet microfluidic platform for high-throughput analysis of platelet aggregation.

ACS Applied Materials & Interfaces, ASAP.

31. Lele Zhou, Zhuo Zhang, Yinliang Tang, Changhao Men, Yuan Luo, Hung-Ta Wang, Yifan Liu*.

Polarity-dependent electro-wetting/dewetting for efficient droplet manipulation.

Physics of Fluids, 2024.

30. Dayin Wang#, Yanan Du#, Ning Wang, Yifan Liu*, Yuan Luo*, Jianlong Zhao.

A novel microfluidic strategy for DNA data random access via droplet digital PCR.

IEEE MEMS, 2024. 

29. Yang Wang, Wenli Gao, Shuo Yang, Qiaolin Chen, Chao Ye, Hao Wang, Qiang Zhang, Jing Ren, Zhijun Ning, Xin Chen, Zhengzhong Shao, Jian Li, Yifan Liu, Shengjie Ling.*

Humanoid Intelligent Display Platform for Audiovisual Interaction and Sound Identification.

Nano-Micro Letters, 2023, 15 (1), 221.

28. Qingyuan Shi#, Jie Li#*, Chunchen Liu#, Xuanpei Zhai, Long Chen, Ye Zhang, Dezhi Feng, Rong Zhang, Jian Li, Shengjie Ling, Lei Zheng*, Yuan Luo*, Yifan Liu*.

Biosensors and Bioelectronics (IF 12.5), 2023, 241, 115702. （上科大新闻报道、分析人新闻报道）

27. Zhirui Dong, Ruoxuan Peng, Yuehua Zhang, Yicheng Shan, Wang Ding, Yifan Liu, Jian Li, Mingdong Zhao, Li-Bo Jiang*, Shengjie Ling*.

Tendon Repair and Regeneration Using Bioinspired Fibrillation Engineering That Mimicked the Structure and Mechanics of Natural Tissue.

ACS Nano (IF 18.0), 17 (18), 17858-17872, 2023. 

26. Jinling Zhu, Chenchen Dai, Hailiang Yang, Yicheng Shan, Jing Ren, Yifan Liu, Jian Li, Wenshuai Chen*, Junrui Liang*, and Shengjie Ling*.

IntelliSense bio-iontronics battery for early warning of geological seepage.

ACS Sensors (IF 9.6), 2023.

25. Long Chen#, Yi Zhao#, Jie Li#, Chenwei Xiong, Yi Xu, Chengren Tang, Rong Zhang, Jingwei Zhang*, Xianqiang Mi*, Yifan Liu*.

Exceeding 80% efficiency of single-bead encapsulation in microdroplets through hydrogel coating-assisted close-packed ordering.

Analytical Chemistry (IF 8.0), 2023, 95, 23, 8889 (supplementary cover). (分析人新闻报道)

24. Ruoxuan Peng#, Fang Ba#, Jie Li#, Jiayi Cao, Rong Zhang, Wan-Qiu Liu, Jing Ren,  Yifan Liu*, Jian Li* and Shengjie Ling*.

Embedding living cells with a mechanically reinforced and functionally programmable hydrogel fiber platform.

Advanced Materials (IF 30.8), 2023. （上科大新闻报道）

23. Yilin Chen, Wan-Qiu Liu, Xiao Zheng, Yifan Liu, Shengjie Ling, Jian Li*.

Cell-Free Biosynthesis of Lysine-Derived Unnatural Amino Acids with Chloro, Alkene, and Alkyne Groups.

ACS Synthetic Biology (IF 5.2), 2023,12, 4, 1349-1357.

22. Fang Ba, Xiangyang Ji, Shuhui Huang, Yufei Zhang, Wan-Qiu Liu, Yifan Liu, Shengjie Ling, Jian Li*.

Engineering Escherichia coli to utilize erythritol as sole carbon source.

Advanced Science (IF 17.5), 2023 Mar 20:e2207008.

21. Hao Zhang, Leitao Cao, Jian Li, Yifan Liu, Zhuochen Lv, Jing Ren*, Zhengzhong Shao*, and Shengjie Ling*.

Dual physically crosslinked silk fibroin ionelastomer with ultrahigh stretchability and low hysteresis.

Chemistry of Materials (IF 10.5), 2023.

20. Yuan Luo#*, Zhen Cao#*, Yifan Liu#*, Rong Zhang, Shijia Yang, Ning Wang, Qingyuan Shi, Jie Li, Shurong Dong, Chunhai Fan and Jianlong Zhao.

The emerging landscape of microfluidics applications in DNA data storage.

Lab on a Chip (IF 7.5), 2023 (Lab Chip Hot Article 2023).（RSC英国皇家化学会新闻报道、微流控新闻报道）

19. Xiaorong Pan#, Jie Li#, Wei Li, Haofei Wang, Nela Durisic, Zhenyu Li, Yu Feng, Yifan Liu, Chun-Xia Zhao* and Tong Wang*.

Axons-on-a-Chip for Mimicking Non-Disruptive Diffuse Axonal Injury Underlying Traumatic Brain Injury.

Lab on a Chip (IF 7.5), 2022 Nov 1:1473-0189. （上科大新闻报道）

18. Yiyun Wang#, Qingyuan Shi#, Yuting Shen#, Yifan Liu* and Fei Gao*.

Passive Photoacoustic Effect.

ARXIV/2206/02548.

17. Luyao Li#, Rong Zhang#, Long Chen, Xintong Tian, Ting Li, Bingchun Pu, Conghui Ma, Xiangyang Ji, Fang Ba, Chenwei Xiong, Yunfeng Shi, Xianqiang Mi, Jian Li*, Jay D. Keasling, Jingwei Zhang* and Yifan Liu*. 

Permeability-Engineered Compartmentalization Enables In Vitro Reconstitution of Sustained Synthetic Biology Systems. 

Advanced Science (IF 17.5), 2022 Sep 30:22203652. (back cover)（AdancedScienceNews新闻报道、BioArtMED新闻报道、MEMS新闻报道、高分子科技新闻报道、微流控新闻报道、上科大新闻报道）

16. Chenguang Wang, Yi Xu, Shuainan Li, Yi Zhou, Qiuling Qiang, Yifan Liu and Xianqiang Mi*. 

Designer tetrahedral DNA framework-based microfluidic technology for multivalent capture and release of circulating tumor cells. 

Materials Today Bio (IF 10.1), 2022 Jul 2;16:100346. 

15. Zhen Cao, Yuxin Ye, Guangyang Li, Rong Zhang, Shurong Dong* and Yifan Liu*. 

Monolithically integrated microchannel plate functionalized with ZnO nanorods for fluorescence-enhanced digital polymerase chain reaction. 

Biosensors and Bioelectronics (IF 12.5), 2022 Oct 1;213:114499. (上科大新闻报道)

14. Chenwei Xiong, Jie Li, Luyao Li, Long Chen, Rong Zhang, Xianqiang Mi* and Yifan Liu*. 

Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes. 

Materials Today Bio (IF 10.1), 2022 May 11;15:100281. (中科院上海微系统所新闻报道)

13. Jie Li#, Rong Zhang#, Ting Li, Qingyuan Shi, Zhangyue Song, Lichun Jiang, Yunfeng Shi, Conghui Ma, Xue Liang, Jingwei Zhang* and Yifan Liu*. 

Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomics. 

BIORXIV/2022/485055.

12. Chen Sun, Leqian Liu, Liliana Pérez, Xiangpeng Li, Yifan Liu, Peng Xu, Eli A. Boritz, James I. Mullins, and Adam R. Abate. 

Droplet-microfluidics-assisted sequencing of HIV proviruses and their integration sites in cells from people on antiretroviral therapy. 

Nature Biomedical Engineering (IF 25.6), 2022 Aug;6(8):1004-1012.

11. Chenwei Xiong#, Boyin Zhang#, Rong Zhang and Yifan Liu*. 

An experimental and numerical study of polyelectrolyte hydrogel ionic diodes: towards electrical detection of charged biomolecules. 

Sensors (IF 3.6), 2021 Dec 10;21(24):8279.

10. Bolin An, Yanyi Wang, Xiaoyu Jiang, Conghui Ma, Mark Mimee, Felix Moser, Ke Li, Xinyu Wang, Tzu-Chieh Tang, Yuanyuan Huang, Yifan Liu, Timothy K. Lu, Chao Zhong. 

Programming Living Glue Systems to Perform Autonomous Mechanical Repairs. 

Matter (IF 19.9), 2020 Dec 2;3(6):2080-92.

9. Conghui Ma, Jie Li, Boyin Zhang, Jingwei Zhang* and Yifan Liu*. 

Hydrogel microparticles functionalized with engineered Escherichia coli as living lactam biosensors. 

Sensors (IF 3.6), 2019 Dec 16;19(24):5556.

8. Yifan Liu，Xuyue Chen, Yiqiu Zhang and Jian Liu. 

Advancing single-cell proteomics and metabolomics with microfluidic techniques. 

Analyst (IF 4.6), 2019 Jan 28;144(3):846-858.

--------------------------------------------------------------------------------------------------------------------

7. Yifan Liu, Yushen Sheng, Lian Duan and Levent Yobas. 

Two-dimensional hydrodynamic flow focusing in a microfluidic platform featuring a monolithic integrated glass micronozzle. 

Applied Physics Letters (IF 3.8), 109, 144101, 2016 

6. Yifan Liu and Levent Yobas. 

Slowing DNA translocation in a nanofluidic field-effect transistor. 

ACS Nano (IF 18.0), 10 (4), 3985-3994, 2016 

5. Yifan Liu and Levent Yobas. 

Microfluidic emulsification through a monolithic integrated glass micronozzle suspended inside a flow-focusing geometry. 

Applied Physics Letters (IF 3.8), 106, 174101, 2015 

4. Yifan Liu and Levent Yobas. 

Label-free specific detection of femtomolar cardiac troponin using an integrated nanoslit array fluidic diode. 

Nano Letters (IF 11.2), 14 (12), 6983–6990, 2014 

3. Yifan Liu and Levent Yobas. 

Label-free electrical quantification of amplified nucleic acids through nanofluidic diodes. 

Biosensors and Bioelectronics (IF 12.5), 50, 78-83, 2013 

2. Yifan Liu and Levent Yobas. 

Cylindrical glass nanocapillaries patterned via coarse lithography (>1 μm) for biomicrofluidic applications. 

Biomicrofluidics (IF 3.3), 6 (4), 046502, 2013 (featured as Editor's Pick) 

1. Zhen Cao#, Luo Yuan#, Yifan Liu, Shuhuai Yao and Levent Yobas. 

Microchannel plate electro-osmotic pump. 

Microfluidics and Nanofluidics (IF 2.9), 13(2), 279–288, 2012 

※申请专利

1. 《一种扩增单细胞内核酸的方法》，专利申请号：202010895311.4

2. 《一种制备磁性聚合物微球的方法及其应用》，专利申请号：202110655120.5，已获得专利授权许可。

3. 《微生物单细胞全基因组扩增及测序文库构建方法》，专利申请号：202210572804.3

4. 《一种聚电解质水凝胶离子二极管及其制备方法和其在核酸检测中的用途》，专利申请号：2022102796706

5. 《一种液滴微流控芯片》，专利申请号：202211124391.9

6. 《一种单细胞基因组三代测序文库的构建方法》，专利申请号：2023106098125

7. 《一种微流控芯片及其应用》，专利申请号：202310464191.6

8. 《一种基于组合荧光编码的液滴装置的细胞电穿孔方法》，专利申请号：202310348184.X

9. 《一种基于超低压数字微流控装置的细胞电穿孔方法》，专利申请号暂无

10.《一种乳液中的可控细胞裂解方法》，专利申请号：202311246397.8

11.《一种月牙微球在数字聚合酶链式反应的应用》，专利申请号：202311242721.9

12.《一种月牙微球在构建单细胞转录组文库的应用》，专利申请号：202311242720.4

13.《一种单细胞全基因组测序建库方法》，专利申请号：202310610886.0

课题组相册

期刊封面

微液滴模块

本组成员

当前成员

	杜亚楠 2019级 博士研究生  安徽大学，应用化学，学士		周乐乐 2021级 博士研究生 上海理工大学，材料成型及控制工程，学士
	职卫良 2023级 博士研究生 上海科技大学，生物学，硕士 中国药科大学，生物工程，学士		翟轩培 2022级 硕士研究生 福州大学，数学与应用数学物理，学士
	肖攀哲 2022级 硕士研究生 山西大学，电子信息科学与技术，学士		崔宇晨 2022级 硕士研究生 华中农业大学，生物科学国家基地班，学士
	张剑 2022级 硕士研究生 西北农林科技大学，林学，学士		朱文杰 2022级 硕士研究生 重庆科技学院，应用化学，学士
	饶思翔 2023级 硕士研究生 上海科技大学，材料科学与工程，学士		李梦真 2023级 硕士研究生 西北大学，生物科学，学士
	童佳伟 2024级 硕士研究生 上海交通大学，生物技术（生物信息试点班），学士		胡宙声 2024级 硕士研究生 华中农业大学，生物工程，学士

已毕业成员

刘晨曦 2020届 本科毕业生 去向：香港中文大学 博士研究生

张博茵 2021届 硕士毕业生 去向：IT行业

李璐瑶 2022届 硕士毕业生 去向：生物医药行业

熊陈玮 2022届 硕士毕业生 去向：香港城市大学 博士研究生

施清源 2023届 硕士毕业生 去向：生物医药行业

朱玥玮 2023届 硕士毕业生 去向：生物医药行业

朱佳伟 2023届 硕士毕业生 去向：生物医药行业

饶思翔 2023届 本科毕业生 去向：留组攻读研究生

洪诚开 2023届 本科毕业生 去向：上海交通大学 硕士研究生