李健

时间:2018-03-01浏览:1016设置

李健课题组介绍


课题组长
李健,助理教授、研究员
通讯地址:物质学院3号楼210A室
电子邮件:lijian@shanghaitech.edu.cn 
2013年9月毕业于德国柏林工业大学(Technischen Universität Berlin),获得自然科学博士学位(Dr. rer. nat.);随后于2013年12月至2017年8月在美国西北大学(Northwestern University)从事博士后研究;2017年9月加入上海科技大学物质科学与技术学院,任助理教授、研究员。

研究介绍




研究组主要利用无细胞合成生物学(Cell-free Synthetic Biology)等技术手段开展在蛋白、药物及健康等方面的研究工作。主要研究内容包括:

1. 新型无细胞蛋白合成体系的开发和利用;

2. 无细胞生物合成具有重要工业和药用价值的蛋白质;

3. 天然产物药物(如非核糖体多肽类抗生素)的生物合成、发现与开发;

4. 新型蛋白质生物材料的设计、开发与应用。




课题组长期诚聘博士后,欢迎对无细胞合成生物学等前沿生物技术感兴趣的硕/博士研究生、本科生、外访生加入!有意者请直接邮件联系。



发表文章
(*Corresponding author, #Co-first author)


21. Li J*, Zhang L, Liu W. Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products. Synthetic and Systems Biotechnology, 2018, 3, 83–89


20. Wang H, Li J*, Jewett MC*. Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements. Synthetic Biology, 2018, 3, ysy003


19. Casini A, Chang FY, Eluere R, King A, Young EM, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Espah Borujeni A, Ryu MH, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O'Connor S, Medema MH, Fischbach M, Jewett MC, Voigt CA, Gordon DB. A pressure test to make 10 molecules in 90 days: external evaluation of methods to engineer biology. Journal of the American Chemical Society, 2018, 140, 4302–4316


18. Li J, Wang H, Jewett MC. Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields. Biochemical Engineering Journal, 2018, 130, 29–33


17. Li J, Wang H, Kwon YC, Jewett MC. Establishing a high yielding Streptomyces-based cell-free protein synthesis system. Biotechnology and Bioengineering, 2017, 114, 1343–1353 (Video Highlighted)


16. Goering AW#, Li J#, McClure RA, Thomson RJ, Jewett MC, Kelleher NL. In vitro reconstruction of nonribosomal peptide biosynthesis directly from DNA using cell-free protein synthesis. ACS Synthetic Biology, 2017, 6, 39–44


15. Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, Jewett MC. Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase. Biotechnology Journal, 2016, 11, 212–218 (Front Cover)


14. Feng J, Yu S, Li J, Mo T, Li P. Enhancement of the catalytic activity and stability of immobilized aminoacylase using modified magnetic Fe3O4 nanoparticles. Chemical Engineering Journal, 2016, 286, 216–222


13. Li H, Yang Q, Li J, Gao H, Li P, Zhou H. The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China. Scientific Reports, 2015, 5, 17056


12. Moatsou D#, Li J#, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK. Self-assembly of temperature-responsive protein-polymer bioconjugates. Bioconjugate Chemistry, 2015, 26, 1890–1899 (Front Cover)


11. Li J*, Jaitzig J, Lu P, Süssmuth RD, Neubauer P. Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations. Microbial Cell Factories, 2015, 14, 83


10. Li J, Zhao Z, Mo T, Wang L, Li P. Immobilization of aminoacylase on electrospun nanofibrous membrane for the resolution of DL-theanine. Journal of Molecular Catalysis B: Enzymatic, 2015, 116, 24–28


9. Li J*, Jaitzig J, Theuer L, Legala OE, Süssmuth RD, Neubauer P. Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli. Journal of Biotechnology, 2015, 193, 16–22


8. Li J*, Neubauer P. Escherichia coli as a cell factory for heterologous production of nonribosomal peptides and polyketides. New Biotechnology, 2014, 31, 579–585


7. Li J#, Jaitzig J#, Hillig F, Süssmuth RD, Neubauer P. Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation. Applied Microbiology and Biotechnology, 2014, 98, 591–601


6. Jaitzig J#, Li J#, Süssmuth RD, Neubauer P. Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli. ACS Synthetic Biology, 2014, 3, 432–438


5. Reitz C, Li J, Neubauer P. Recombinant protein production: a comparative view on host physiology (meeting report). New Biotechnology, 2013, 30, 405–409


4. Li P, Wu C, Wu Q, Li J, Li H. Biosynthesis of different morphologies of CaCO3 nanomaterial assisted by thermophilic strains HEN-Qn1. Journal of Nanoparticle Research, 2009, 11, 903–908


3. Li J, Li P, Liu F. Production of theanine by Xerocomus badius (mushroom) using submerged fermentation. LWT-Food Science and Technology, 2008, 41, 883–889


2. Li P, Wan X, Zhang Z, Li J, Shen Z. A novel assay method for theanine synthetase activity by capillary electrophoresis. Journal of Chromatography B, 2005, 819, 81–84


1. Li P, Li J, Wu C, Wu Q, Li J. Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnology, 2005, 16, 1912–1917



本组成员

刘晚秋 助理研究员

2015-2017 复旦大学 博士后
2009-2015 中科院微生物所 博士

邮箱:liuwq@shanghaitech.edu.cn

张凌凯 硕士研究生

2013-2017 湘潭大学 学士

邮箱:zhanglk@shanghaitech.edu.cn

乔若频 2014级本科生

上海科技大学

邮箱:qiaorp@shanghaitech.edu.cn

陈铭哲 2015级本科生

上海科技大学

邮箱:chenmzh@shanghaitech.edu.cn

刘旸 2016级本科生

上海科技大学

邮箱:liuyang2@shanghaitech.edu.cn


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